STEAP1B基因敲除质粒

pLenti-STEAP1B-sgRNA (STEAP1B基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的STEAP1B基因敲除的质粒(L13715 pLenti-STEAP1B-sgRNA)、慢病毒(L13716 STEAP1B Knockout Lentivirus)、HEK293T细胞(L13717 STEAP1B Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L13718 STEAP1B Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L13719 STEAP1B Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

STEAP1B基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	STEAP1B	256227	BC066301	NM_001164460

 

About the gene
Official Symbol	STEAP1B
Previous Symbol	-
Official Full Name	STEAP family member 1B
Synonyms	-
Location	7p15.3
Gene Type	protein-coding gene
Uniprot ID	Q6NZ63
Pathway/Library	Lung Cancer Growth Related Genes Library
Gene Summary	The six transmembrane epithelial antigen of the prostate 1 (STEAP1) is overexpressed in prostate cancer, and other malignant tumors with slightly less intensity. Regarding normal tissues, STEAP1 expression is almost restricted to prostatic cells preferentially located on the plasma membrane of epithelial cells, particularly on cell-cell junctions, and to a lesser extend dispersed on the cytoplasm. STEAP1 appears to function as a transporter or ion channel, taking part on inter- and intracellular communication, possibly regulating cell proliferation and invasiveness.The STEAP1 as a potential immunotherapeutic target as well as a biomarker, emphasizing its clinical relevance. Besides STEAP1 gene, another related gene, STEAP1B, is encoded by the human genome. This gene is located at a different arm of the same chromosome as STEAP1, and may originate two different transcripts, STEAP1B1 and STEAP1B2. STEAP1 is regulated by androgens and estrogens on the LNCaP cell line.