SPSB4基因敲除质粒

pLenti-SPSB4-sgRNA (SPSB4基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的SPSB4基因敲除的质粒(L10675 pLenti-SPSB4-sgRNA)、慢病毒(L10676 SPSB4 Knockout Lentivirus)、HEK293T细胞(L10677 SPSB4 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L10678 SPSB4 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L10679 SPSB4 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

SPSB4基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	SPSB4	92369	BC008324	NM_080862

 

About the gene
Official Symbol	SPSB4
Previous Symbol	-
Official Full Name	splA/ryanodine receptor domain and SOCS box containing 4
Synonyms	SSB-4
Location	3q23
Gene Type	protein-coding gene
Uniprot ID	Q96A44
Pathway/Library	Ubiquitin Ligases Genes Library
Gene Summary	Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21199876, PubMed:15601820). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876). Diminishes EphB2-dependent cell repulsive responses by mediating the ubiquitination and degradation of EphB2/CTF2 (PubMed:28931592). Regulates cellular clock function by mediating the ubiquitin/proteasome-dependent degradation of the circadian transcriptional repressor NR1D1 (PubMed:26392558). SPSB4_HUMAN,Q96A44