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- MedChemExpress(MCE)
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- 2025年12月05日
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文献和实验the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant. Add 1ml of column buffer containing 0.5 mM GTP to each tube
into new 30 ml ultracentrifuge tubes. 8. Balance the tubes and incubate for 45 min in a water bath at 34°C (the solution at this stage is pinkish). During the incubation, warm the Ti70 rotor with tap water and set
Recycling Tubulin We "recycle" tubulin fractions stored at -80¡C after the PC column and store the recycled tubulin in small aliquots for day-to-day use. We generally store recycled tubulin in Injection Buffer (IB) without free GTP
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