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文献和实验为存在于细菌中 2型拓扑异构酶的一种。可将双螺旋 DNA的股解开导入负的超螺旋(使 W数变负)的独特的酶。大肠杆菌的促旋酶为由 2种多肽链各二分子所形成的四聚体,其中的二种肽链则分别由基因 Gyr A和 Gyr B所产生,分子量各为 11.5万和 9.5万,拓扑学异构化是通过双链的暂时切断而进行,作为其中间产物,在四核苷酸对分离的地方形成裂缝,酶分子与 3′ -OH游离端和 5′ -磷酸末端形成共价键的形式。此酶的催化作用有是:在 ATP的存在下,使弛缓型的闭环状 DNA
Plasmid DNA Supercoiling by DNA Gyrase
DNA gyrase catalyzes DNA supercoiling in a reaction coupled to ATP hydrolysis (1 ). The enzyme has been found in many eubacterial species and is unique among the topoisomerases in promoting negative supercoiling of DNA (2 ,3 ). A variety
Quinolone Interactions with DNA and DNA Gyrase
our understanding of the established structure-activity relationship of that class of compounds. The method has been best exemplified by the investigations of mode of action of quinolone antibacterials that target the bacteria-specific type II DNA topoisomerase, DNA
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