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- Profoldin
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文献和实验WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
; Hybridization mix recipe (50 ml): 0.5 g Boehringer Block 25 ml formamide 12.5 ml 20X SSC, pH 7 Heat to 65 °C for about 1 hr. Once dissolved add: 6 ml H2O 5ml 10mg/ml torula RNA
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
and 25% methanol series in PBSw. Incubate each step for 5 min. on ice. Wash 3 times for 5 min. with PBSw on ice. Change to 1ml of 4.5 µg/ml Proteinase K in PBSw. Incubate for 3 min for 6dpc at RT, 5 min for 7.5dpc, 7 min for 8.5 dpc
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos.
after proteinaseK treatment. Prepare hybridization solution. For 50 ml of hybridization solution dissolve; Hybridization mix recipe (50 ml): 0.5 g Boehringer Block 25 ml formamide 12.5 ml 20X SSC, pH 7
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