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文献和实验Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
Large Scale Tubulin Preparation
Large Scale Tubulin Preparation Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described
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