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文献和实验Co-Expression of human DNA primase p49-p58 subunits
primase subunits The most important thing in doing this purification is to make sure that EVERYTHING is kept cold.Prepare all buffers ahead of time and pre-chill them.Pre-chill rotor,centrifuge tubes,falcon tubes,etc.It's best
, A.R., Barnum, J.E., Harmon, L.S., DeLuca, J.G., and Nichols, W.W. 1994. Rapid DNA degradation in primary rat hepatocytes treated with diverse cytotoxic chemicals: Analysis by pulsed‐field gel electrophoresis and implications for alkaline elution assays
DNA Fragmentation Assays for Apoptosis
Protocol II: DNA Fragmentation Assay via Dipheylamine In 24-wells plate, incubate 5 X 106 targets with desired number of effectors. After incubation, transfer the samples to 15ml tubes, centrifuge for 30 s at 1500g, and resuspend in 5ml
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