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999
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500 assays
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
RAT/MOUSE GROWTH HORMONE ELISA KIT
should be approximately 2.0-3.2, or not to exceed the capability of the plate reader used. 3. INTERPRETATION 1) The assay will be considered accepted when all Quality Control values fall within the calculated Quality Control Range. If any QC’s fall
Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
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