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- Profoldin
- DSA100KP
- 2025年07月12日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
-radioactive labeling Mix (-DIG-dUTP) is also included in the kit as an alternative labeling of DNA (DIG-dUTP is not included). COMPONENTS OF THE KIT1.Klenow Fragment, exo-, 5u*/µl: 15µl (30µl) of the enzyme solution in buffer containing 50% glycerol.2
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