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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
Commonly used restriction enzymes and assay buffers Common Assay Incub. Recognition Enzyme isoschizomers buffer temp. site Cloning sites Aat II med 37 GACGT/C Aat II Acc
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