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文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Cellular lysis solution ChIP diluent Cloning vector The vector used should have enzyme overhangs similar to those generated in the 3C assay. Such vectors can be custom-constructed or obtained commercially. For example, for cloning
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
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