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文献和实验Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses
Sauer:RNA Purification from E. coli
quantitation Images of E. coli RNAs E. coli RNAs resolved in a denaturing gel and subsequently stained with SYBR Green II dye (Invitrogen) Left: Four samples of RNA resolved so that the smallest and largest RNAs are visible. Middle
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
gently but throughly by inverting and rotating tube 4-6 times to obtain a cleared lysate. A 2 min incubation at room temperature may be necessary. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II
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