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文献和实验Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
The E. coli JS200 strain carries a temperature-sensitive allele of DNA polymerase I that renders this strain conditional lethal. Growth under restrictive conditions is restored by small amounts of DNA polymerase activity. Even mutants
Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses
Strategies to Optimize Protein Expression in E. coli
that Influence Expression and Solubility III. Properties of the Vector that Influence Expression and Solubility IV. E. coli Host Strains that aid Expression of Heterologous Proteins V
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