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- Profoldin
- DNAB100KH
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 规格：
100 assays
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
2.Quantitate Protein Concentration Quantitate proteins in 15 ml aliquots of lysates (in duplicate)by using a Bradford assay (I have been using the Biorad kit),using BSA as standard.Don't forget to add 15 ml LB to each BSA sample and to the blank tube
Radioactive DNA Fragmentation Assay
DESCRIPTION of the method: The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agents, e.g. with TNF-alpha or anti-Fas antibody (IPO-4).
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