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文献和实验The production of monoclonal antibody by hybridoma
of ~5ug of antigen in PBS i.p. or intravenously in tail vein of the mouse (with high titer already determined). 1. Spin down myelomas and wash with 30 ml serum free media (CMNS has glutamine). Use tabletop centrifuge at 850
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
of finding a clone within a given library? The exact probability of having any given DNA sequence in the library can be calculated from the equation N = ln(1 -P) /ln(1 - f) P is the desired probability f
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