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文献和实验Quantification of Mouse IL-6 and TNF- mRNA Using Xplore Assays
to measure both concentration of the free cytokine in solution and cytokine mRNA in the cytokine-expressing cells. Traditional methods of examining gene expression, such as as Northern blots, reverse transcriptase polymerase chain reaction (RT-PCR), and RPA
Free-Hand Bisection of Mouse Oocytes and Embryos
functions and by Western blot and genetic analysis (e.g., RT-PCR). Here we describe a detailed protocol for the free-hand bisection of mouse zona pellucida -free oocytes and embryos on an agar layer using a glass needle.
Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy
may be tolerated at one institution, the impact on the studies at another may demand clean animals. The importance of a well-established veterinary health surveillance program and a list of organisms to be excluded from the facility, i.e., a Specific Pathogen Free
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