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文献和实验Assay of mtDNA Polymerase from Human Tissues
3 ). Figure 1 illustrates some of the protein components that participate in mitochondrial DNA replication. A principal component of the mtDNA replisome is the mtDNA polymerase γ. This enzyme is found as an αβ heterodimer and as an α monomer
Cytotoxins Directed at Interleukin-4 Receptors as Therapy for Human Brain Tumors
Interleukin-4 (IL-4) is a pleiotropic immune regulatory cytokine that has been extensively studied in the last decade. Activated T-lymphocytes, mast cells, and basophils (1 –3 ) produce it. Consistent with the pleiotropic nature
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
MEF-Conditioned Medium (MEF-CM) 1) Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C or for 1 hour at room temperature. For MEF-CM generation, a T-175 flask
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