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文献和实验【翻译】Development trends for monoclonal antibody cancer therapeutics
as IgG2a, four mAbs were IgG3, four were IgG1, and one mAb was known only to be IgG. No correlation was observed between the isotype of the naked murine mAbs and the available information on the primary reason for termination of development (for example
The production of monoclonal antibody by hybridoma
of ~5ug of antigen in PBS i.p. or intravenously in tail vein of the mouse (with high titer already determined). 1. Spin down myelomas and wash with 30 ml serum free media (CMNS has glutamine). Use tabletop centrifuge at 850
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
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