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文献和实验Western Blotting with Monoclonal Ant
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. Polyacrylamide Gel
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
proteins) are relatively difficult to investigate by standard yeast two?hybrid methods either because they are retained at cellular membranes or they activate the system in the absence of a true protein interaction. The membrane?based yeast two?hybrid (MbY
Western Blotting with Monoclonal Antibodies
Sample Preparation For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). Aspirate excess PBS. Add 1ml
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