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文献和实验by a non-relevant immunoglobulin (i.e, normal serum for polyclonal antibodies, control mouse ascites fluid for ascites, and isotype controls for purified mouse monoclonal antibodies). 3) Proteolytic digestion can occur when cells are lysed and contents
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
by a non-relevant immunoglobulin (i.e, normal serum for polyclonal antibodies, control mouse ascites fluid for ascites, and isotype controls for purified mouse monoclonal antibodies). 3) Proteolytic digestion can occur when cells are lysed and contents
Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
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