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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Immunohistochemisty-Fluorescence Protocol
and Secondary Reagents Biotinylated donkey anti-goat IgG (Jackson Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 (Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 (Caltag Lab Catalog # M32001)
which contain two identical heavy chains, and two identical light chains. The stem of the 'Y' comprises the Fc (constant) domain , and the 'arms' of the 'Y' comprise the Fab (variable) domains . Antigens bind to the complementarity
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