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文献和实验【申请上传】Current Protocols in Immunology
Production Unit 2.1 Enzyme-Linked Immunosorbent Assays Unit 2.2 Isotype Determination of Antibodies Unit 2.3 Double-Immunodiffusion Assay for Detecting Specific Antibodies Section II Production of Antibodies Unit 2.4 Production of Polyclonal Antisera
. Typical host animals include mouse, chicken, rabbit, goat, sheep, horse and occasionally, human. After an initial immunization, followed by one or more booster shots, the B lymphocytes of the host animal may produce antibodies directed
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
rabbit polyclonalsFRP-1 antisera directed against either the full-lengthprotein or peptide segments; alternatively, one can use commerciallyavailable reagents such as a goat polyclonal antiserum(sc-7425; Santa Cruz Biotechnology, Santa Cruz, CA
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