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文献和实验Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells
Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control. Hoechst 33342 stock solution (1mg/ml) (see recipe) 12 X 75 mm culture tubes Vortex
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
Introduction Green fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien, 1996; Tsien, 1998). The validation and interpretation of these data
GFP gene encoding Green Fluorescent Protein mutant p11 URA3 URA3 gene from S. cerevisiae tet Tetracycline resistance gene res Tn3 site for resolution
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