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文献和实验Direct Immunofluorescence Labeling
sera: Usually, purified mouse IgM or IgG of the same subclass as the test antibodies and conjugated to the same fluorochrome. One usually purchases them from the same manufacturer to get a similar fluorochrome-to-protein ratio as the test antibodies.
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
. You can see how complicated this can become... The best solution for simultaneous labeling is get all direct conjugates, if at all possible. Then see our our protocol for direct labeling. Materials Primary Antibodies: Test antibody: Usually mouse monoclonal IgG or IgM
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