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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Clone Genes From a Phage Library
may be amplified if you wish, but this should not be necessary. It was made by cutting the genomic DNA with Tsp509 I at several different concentrations and size-selecting pieces from 4 to 8-9 kb from each digestion to ligate into the Lambda ZapII vector (at Eco RI
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes, 4 times at room temperature. Develop membranes by diluting 33 µl NBT and 66µl BCIP in 10
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