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文献和实验One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely
Polyclonal and Monoclonal Antibodies in Clinic
the highly evolved machinery of the immune system to produce structurally and functionally complex molecules such as antibodies from a single B clone, heralding the era of monoclonal antibodies. Most of the antibodies currently in the clinic, target
Use of the B.D. FACS or Calibur Flow Cytometers
. no BrdU added to the culture dish, or a knockout cell line). If this is not available then cells stainined with pre-immune serum + secondary or flourescently labeled isotype antibodies should be used. If multicolor flow is being used multiple isotype
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