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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
DNA gyrase and DNA topoisomerase (topo) IV are the bacterial targets of coumarin and quinolone antimicrobial agents. Widespread resistance to clinically important antibiotics such as beta-lactams and macrolides has stimulated the development
Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses
. Promega has developed Gel Shift Assay Systems that contain target oligonucleotides, control extracts containing DNA -binding proteins, binding buffer and reagents for phosphorylating oligonucleotides. The Core System includes an E. coli extract
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