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文献和实验Coomasie Blue Staining of Protein Gels
destaining unit (Optional) Procedure Place a gel prepared as in Exercise 4.2 in at least 10 volumes of Coomasie Blue staining solution for 2-4 hours. Rock gently to distribute the dye evenly over the gel.
. Colloidal iron stains are unpredictable. Alcian blue stains are simple, but have a lot of background staining. Stains for biogenic amines Cells that produce polypeptide hormones, active amines, or amine precursors (epinephrine, norepinephrine
Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
The identification of proteins using preparative gel electrophoresis and mass spectrometry requires reversible staining of relatively thick (1–1.5 mm) polyacrylamide gels. We have found that staining with colloidal Coomassie brilliant blue G
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