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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
editing using sequence‐specific nucleases. Sequence‐specific nucleases are composed of a domain that binds to the target gene via zinc‐finger or TAL effector‐based DNA recognition motifs. The DNA binding part is fused to the nonspecific nuclease domain
and Rep40) and one unspliced (Rep78 and Rep52) mRNA. Within the rep ORF, several functional domains have been identified and are indicated at the top. These include the DNA binding (DB), trs (Y), NTP binding pocket (NTP), nuclear localization domain (NLS
Introduction of human 14-3-3 proteins
terminus of GPIb-alpha,as indicated by antibody inhibition and direct binding of 14-3-3zeta to synthetic GPIb-alpha cytoplasmic domain peptides.The 14-3-3zeta binds to recombinant wild type GPIb-IX but not to the GPIb-alpha mutants lacking C-terminal
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