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文献和实验Preparation of Polyclonal Antibodies to Retinoid Receptors
with antibodies to retinoic acid receptor a (RARα) enabled visualization of the promyelocyte-retinoic acid receptor (PML-RAR) fusion oncoprotein in cells from acute promyelocytrcleukemia patients (5 , 6 ) This chapter describes basic protocols for polyclonal
Polyclonal Antibody Production
to the pre-bleed, but get at least 20 mL per rabbit, with 40 mL being the upper limit for an adult rabbit (any more and they get anemic). Monitoring of Titer The quality of the antibodies in serum (humoral immune response) of the bleeds
Detection of Known Allergen-Specific IgE Antibodies by Immunological Methods
are incubated with the respective diluted serum sample (approximately 25 μL serum required) and bound IgE antibodies are detected with a secondary horseradish peroxidase (HRP) labelled anti-human-IgE antibody via chemiluminescence. The measurement
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