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文献和实验Western Blotting with Monoclonal Ant
Sample Preparation For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
【翻译】Development trends for monoclonal antibody cancer therapeutics
as IgG2a, four mAbs were IgG3, four were IgG1, and one mAb was known only to be IgG. No correlation was observed between the isotype of the naked murine mAbs and the available information on the primary reason for termination of development (for example
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