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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
【翻译】Development trends for monoclonal antibody cancer therapeutics
were not available, general trends could be discerned. Of the unmodified mAbs with a known isotype, 77 (95%) were IgG, whereas 4 (5%) were IgM, which function primarily through CDC. The majority (80%) of the 61 IgG mAbs with human Fc regions (murine mAbs excluded
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