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5 µg
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文献和实验our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
Characterization of Calcium Channel Binding
Figure 1.25.1 (A ) Saturation binding of [3 H]PN200‐100 to L‐type calcium channels in mouse heart membrane preparation ( n = 2). (B ) Scatchard analysis of the specific binding data: K d = 54.9 pM and B max = 116.4 fmol/mg protein
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
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