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文献和实验Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and SELDI-TOF
Cardiac troponin I (cTnI) is a key regulator of cardiac muscle contraction. Upon myocardial cell injury, cTnI is lost from the cardiac myocyte and can be detected in serum, in some cases with specific disease-induced modifications, making
Application of MicroRNA in Cardiac and Skeletal Muscle Disease Gene Therapy
have demonstrated that miRNAs are essential for normal mammalian development and organ function. Deleterious changes in miRNA expression play an important role in human diseases. We and others have previously reported several muscle-specific miRNAs, including miR
Expression of Human eNOS in Cardiac and Endothelial Cells
In this chapter we provide a detailed technique-focused protocol for expression of human endothelial nitric oxide synthase (eNOS) gene delivered by replication-deficient adenovirus. It includes construction of recombinant plasmid with human
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