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文献和实验Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing
Class I human leukocyte antigen (HLA-I) alleles have been described as themost polymorphic human antigens (1 -4 ). To date, there are more than 500 HLA- I alleles which have been officially assigned by the World Health Organization
Expression of Human eNOS in Cardiac and Endothelial Cells
In this chapter we provide a detailed technique-focused protocol for expression of human endothelial nitric oxide synthase (eNOS) gene delivered by replication-deficient adenovirus. It includes construction of recombinant plasmid with human
. Here, we describe a delivery system based on the yeast Saccharomyces cerevisiae that allows the delivery of functional messenger RNA to mammalian antigen-presenting cells such as human dendritic cells. Further, we present a method to prove antigen processing and presentation
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