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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
Nucleic acid hybridization assays using cloned DNA probes
) probes to discriminate between alleles differing at a single nucleotide position The general procedure of dot-blotting involves taking an aqueous solution of target DNA, for example total human genomic DNA, and simply spotting
Hybridization of High Density Arrayed BAC Nylon Filter Blots
chain reaction Thaw LB/10% glycerol stock plates containing Unigene clones. To a 96 well PCR, thin walled plate containing 99µl of sterile ddH2 O per well, add 1µl of Unigene culture taken from the bottom of the glycerol stock wells
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