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文献和实验Generation and Functional Analysis of Zinc Finger Nucleases
on homologous recombination, which can be stimulated by the creation of a targeted DNA double-strand break (DSB) near the site of the desired recombination event. Artificial nucleases containing zinc finger DNA-binding domains have provided important proofs
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
Wiley & Sons, Inc. Keywords: pronucleus injection; gene targeting; mouse mutant; zinc?finger nuclease; TAL nuclease; homologous recombination
A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
the wild-type and recombinant viruses at appropriate dilutions are used to infect cell monolayers of human TK− cells, and then overlaid with growth medium containing agarose and 5-BrdU to prevent spreading of recombinant virus plaques and inhibit
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