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文献和实验Analysis of the Protein S Gene in Protein S Deficiency
Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker
Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared
Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
Antibodies of the G class can be conveniently purified, even at large scale, by affinity chromatography using immobilized protein A or G. Because specific and cost-effective ligands are not available, scaling up purification of immunoglobin
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