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文献和实验Genetic Analysis of a Highly Homologous Gene Family: The Killer Cell Immunoglobulin-Like Receptors
Human natural killer (NK) cells express an array of inhibitory and activating receptors some of which interact with major histocompatibility complex (MHC) class I molecules. Expression of these receptors varies at a clonal level generating
cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
Overview of Receptor Allosterism
modulators on orthosteric ligand dissociation kinetics. (A ) Enhancement of the dissociation rate of [3 H]yohimbine from the human α2A receptor expressed in CHO cell membranes by the modulator 5‐( N ‐ethyl‐ N ‐isopropyl)‐amiloride (EPA). Data taken
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