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文献和实验Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
Activation of Nuclear Factor-B
Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
Utilization of Thyroid Transcription Factor-1 Immunostaining in the Diagnosis of Lung Tumors
only in those cells. Thyroid transcription factor-1 (TTF-1) is a 38-kDa homeodomain containing nuclear transcription protein of the Nkx2 gene family. It consists of a single polypeptide of 371 amino acids that is encoded by a single locus (2 ). TTF-1 is expressed
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