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文献和实验The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
The Ipl1/Aurora family of protein kinases are required for accurate chromosome segregation. Because members of this family are often overexpressed in human tumors, they have recently received much attention, both from the academic community
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells
and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell
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