Recombinant Mouse TEK Tyrosine

Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells

are then differentiated along the endothelial lineage using endothelial growth medium supplemented with 40 ng/mL vascular endothelial growth factor (VEGF). The differentiated endothelial population expresses both Fetal Liver Kinase 1 (Flk-1) and VE-Cadherin on the cell

Measurement of Wee Kinase Activity

or in purified preparations. Specific protocols include the production and purification of recombinant Cdc2/Cyclin B substrate, the production of crude subcellular extract fractions, the purification of endogenous or recombinant Wee kinases, Wee kinase assays

Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets

kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration