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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
, Ku. Ku is composed of 70-kDa and approx 80-kDa subunits (called Ku70 and Ku80, respectively) and targets DNA-PKcs to ends of double-stranded DNA. DNA-PKcs is related by amino acid sequence to the phosphatidyl inositol kinase (PI-3 kinase) protein
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells
and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell
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