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文献和实验Peptide microarray technology can be used to identify substrates for recombinant kinases, to measure kinase activity and changes thereof in cell lysates and lysates from fresh frozen (tumor) tissue. The effect of kinase inhibitors
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
family (4 ), however, in vitro DNA-PK acts as a serine/threonine protein kinase (5 –3 ). The physiological substrates of DNA-PK are not known, however in vitro DNA-PK is known to phosphorylate many proteins including hsp90, SV40 large T antigen, serum
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
microarrays per kinase to be probed (e.g., from protocol 2 ) Superblock/0.1% Triton X‐100 (Thermo/Fisher) Kinase to be assayed
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