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文献和实验Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus
Vaccinia virus (VV) has proven to be a very useful tool for the expression and analysis of foreign gene products. The most common method used to produce recombinant viruses involves the insertion of foreign genes into the thymidine kinase (TK
disorders), including translocations. Somatic cell hybrids are constructed by fusing mouse thymidine kinase-deficient 3T3 fibroblasts with human lymphoblastoid cells, as a result of which specific hybrid cells containing only cytogenetically abnormal human
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
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