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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells
and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell
A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
of the gene of interest at the viral thymidine kinase (TK) gene locus results in recombinant viruses with the TK− phenotype, plaque isolates of recombinant viruses are selected in the presence of 5-bromodeoxyuridine (5-BrdU). The conditions chosen
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