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文献和实验Nonradioactive Labeling and lmmunodetection of Recombinant Proteins
, or Western blot can be carried out using enzyme-labeled primary or secondary antibodybased systems or it can be performed using biotmylated primary or secondary antibodies with streptavidin or avidin-enzyme conlugates. Biotin is often the hapten of choice
Approaches to the Identification of Novel Epitopes Christian Brander, and Philip J. R. Goulder Clinical Trials of HIV Vaccines Barney S. Graham Recombinant HIV Sequences: Their Role in the Global Epidemic Martine Peeters The Characterization of HIV
element; MLV = murine leukemia virus; env = envelope; VSV G = vesicular stomatitis virus G protein. The accessory proteins (VIF, VPR, VPU, NEF) of HIV-1 have been deleted. The dashed line indicates the HIV-1 sequences that have been removed from the gag
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