HIV-1 Circulating Recombinant

Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase

Reverse transcriptase (RT) has attracted particular attention as a target enzyme for AIDS chemotherapy, because the enzyme catalyzes a crucial step in the HIV replicative cycle. Effective inhibition of this enzyme prevents the formation

Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)

This chapter describes a straightforward protein purification strategy for the specific separation of insoluble recombinant proteins (so-called “inclusion bodies”) located in the E. coli cytoplasm and their subsequent recovery in form

Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase

assay that differentiates the mitochondrial form from what generally prove to be multiple, and much more abundant, nuclear counterparts. A case in point is the mitochondrial DNA polymerase, pol γ. Pol γ was the third DNA polymerase identified in animal