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- Cedarlane
- CLF1603-TR
- 2025年07月09日
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文献和实验with different antibodies for simultaneous analysis, you may encounter problems choosing secondary antibodies (fluorochrome conjugated antibodies that bind to the primary antibody). For example, if both primary antibodies are mouse IgG 1, what can you do to label
Direct Immunofluorescence Labeling
sera: Usually, purified mouse IgM or IgG of the same subclass as the test antibodies and conjugated to the same fluorochrome. One usually purchases them from the same manufacturer to get a similar fluorochrome-to-protein ratio as the test antibodies.
免疫组化技术-Immunohistochemical Protocols
Incubation in affinity purified indocarbocyanine (Cy3, viewed in red field, 1:1000 in BSA diluent) or fluorescein isothiocyanate (FITC, viewed in green field, 1:100 in BSA diluent) fluorochrome conjugated secondary antibodies (F[ab']2 fragments
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