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文献和实验T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
C in a water bath) for 40 min in 25 mM Tris–HCl (pH 8.5), 1 mM EDTA, and 0.05% SDS (Tris–EDTA–SDS) buffer. Sections are then immunostained with primary and secondary antibodies conjugated with polymer-labeled Horse Radish Peroxidase. Compared
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
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