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文献和实验Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli
and structural studies relating to preclinical drug development. This chapter provides a detailed technical description of the processes that allow the coexpression of various CYP isoforms together with CYP reductase in Escherichia coli and gives some examples
Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
. Fluorometric assays incorporating recombinant human enzymes are ideally suited for this purpose.
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